Composition of human islet cell preparations for transplantation

Cellular composition of islet cell suspensions for transplantation.

A novel method for the assessment of cellular composition and beta-cell viability in human islet preparations.

Current methodologies to evaluate islet cell viability are largely based on tests that assess the exclusion of DNA-binding dyes. While these tests identify cells that have lost selective membrane permeability, they do not allow us to recognize apoptotic cells, which do not yet stain with DNA-binding dyes. Furthermore, current methods of analysis do not discriminate between cell subsets in the p...

-Cell Function Following Human Islet Transplantation for Type 1 Diabetes

Islet transplantation can provide metabolic stability for patients with type 1 diabetes; however, more than one donor pancreas is usually required to achieve insulin independence. To evaluate possible mechanistic defects underlying impaired graft function, we studied five subjects at 3 months and four subjects at 12 months following intraportal islet transplantation who had received comparable ...

Role of Tissue Dissociation Enzymes on Human Islet Yield Intended for Clinical Islet Cell Transplantation

Gopalakrishnan Loganathan1, Venugopal Subhashree2 and Appakalai N Balamurugan1* 1Clinical Islet Cell Laboratory, Cardiovascular Innovation Institute, Department of Surgery, University of Louisville, Kentucky, USA 2School of Biosciences and Technology, VIT University, Vellore, India *Corresponding author: Appakalai N Balamurugan, Ph. D Clinical Islet Cell Laboratory, Center for Cellular Transpla...

Islet cell hormonal responses to hypoglycemia after human islet transplantation for type 1 diabetes.

Islet transplantation can eliminate severe hypoglycemic episodes in patients with type 1 diabetes; however, whether intrahepatic islets respond appropriately to hypoglycemia after transplantation has not been fully studied. We evaluated six islet transplant recipients, six type 1 diabetic subjects, and seven nondiabetic control subjects using a stepped hyperinsulinemic-hypoglycemic clamp. Also,...